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Ships within 48 hours · Estimated delivery Aug 17 - Aug 22
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Description
Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA KitThe Rat ALPL (Alkaline Phosphatase, Liver Bone Kidney) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat ALPL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALPL and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
The Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ALPL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALPL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALPL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ALPL. You can calculate the concentration of Rat ALPL in the samples by comparing the OD of the samples to the standard curve.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
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