Human C5A (Complement Component 5a) QuickTest ELISA Kit

Human C5A (Complement Component 5a) QuickTest ELISA KitThe Human C5A (Complement Component 5a) QuickTest ELISA Kit measures C5a in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into

The Human C5A (Complement Component 5a) QuickTest ELISA Kit measures C5a in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains C5a, a capture antibody-C5a-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C5a in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.