fT4 (Free Thyroxine) ELISA Kit
SKU: 32013234418

fT4 (Free Thyroxine) ELISA Kit

Sale price$202.50 Regular price$225.00
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Description

fT4 (Free Thyroxine) ELISA KitThe fT4 (Free Thyroxine) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with fT4. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to fT4. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate

The fT4 (Free Thyroxine) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with fT4. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to fT4. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 32013234418

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4.1 ★★★★★
Based on 1344 reviews
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L
Verified Purchase
Lisa Jones
Fort Morgan, US
★★★★★ 5
Good for an aggressive chewer
Size: 6 Inch, Color: Bone
My dog loves it. These last a long time. My dog is a Rottweiler/American Bulldog mix, she goes through a lot of toys. So, I try to buy her something that has no stuffing, but will keep her from getting bored. This one has lasted about 6-7 months, the leather is chewed off, but it's still the shape of a bone. I did just order another though, the one she has is a little stinky from her slobber. :-)
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Reviewed in the United States on April 11, 2026
K
Verified Purchase
Karen K.
Carnegie, US
★★★★★ 3
Not as expected
Size: 6 Inch, Color: Bone
I have been looking for these ever since we got a similar one at Sierras for our 1 1/2 yo Cane Corso. She is brutal on chew toys and the first one from the box store held up well. However, this did not hold up as well and she was done with it in less than a week. Still looking for that perfect toy.
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Reviewed in the United States on May 20, 2026
J
Verified Purchase
Jackie
Grantham, US
★★★★★ 5
More durable than expected.
Size: 5 Inch, Color: Raccoon
I was walking through my front room the other day when I noticed a suspicious spot on the floor. It looked like something I should not touch without gloves. As I got closer, I realized what it was. It’s the ‘guts’ from this toy. My dogs (Pit Bull and German Shorthaired Pointer) were each given one. Both are aggressive chewers with their toys. They ATE the faces off these toys and now play with the felt interior. I’m still trying to figure out how they ate through leather while the felt remained intact. Regardless, they still play with them. Now they are basically semi-fuzzy throw toys we can throw in the house because they don’t leave marks on the wall if our aim is off. Sometimes things work out the way they should. :)
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Reviewed in the United States on March 12, 2026
Z
Verified Purchase
ZC
Lake Worth, US
★★★★★ 4
Great with supervision
Size: 6 Inch, Color: Bone
My dogs LOVE these, but removing a star because these apparently are VERY delicious and they will ingest what they rip off if I don’t supervise; also removing a star for how quick they’re able to tear these up. These are like catnip for dogs, I’ll still buy them!
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Reviewed in the United States on September 13, 2025
P
Verified Purchase
Penny Sinclair
Louisville, US
★★★★★ 1
Don't waste your money!!
Size: 5 Inch, Color: Fox
With in minutes my puppy was pulling on the middle fiber stuff. I was worried he would choke/ingest it so I immediately threw it away just like the money I apparently threw away on this unsafe and misleading chew toy.
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Reviewed in the United States on May 29, 2026

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