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Ships within 48 hours · Estimated delivery Jul 12 - Jul 17
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Description
ASM (Acid Sphingomyelinase) ELISA Kit (Small Sample)The ASM (Acid Sphingomyelinase) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human ASM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human ASM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
The ASM (Acid Sphingomyelinase) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASM in the samples is then determined by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
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