VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit

VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2) ELISA KitThis VEGFR 2 (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human VEGFR 2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human VEGFR 2 are added to the micro ELISA plate wells. Human VEGFR 2 in samples (or standards) combines with the coated antibody and HRP linked detection

This VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit uses the sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human VEGFR-2 are added to the micro ELISA plate wells. Human VEGFR-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human VEGFR-2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human VEGFR-2. The concentration of Human VEGFR-2 in the samples is then determined by comparing the OD of the samples to the standard curve.