SKU: 55173751277

CCR4 (Chemokine C-C-Motif Receptor 4) ELISA Kit (Small Sample)

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Description

CCR4 (Chemokine C-C-Motif Receptor 4) ELISA Kit (Small Sample)The CCR4 (Chemokine C C Motif Receptor 4) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human CCR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human CCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate

The CCR4 (Chemokine C-C-Motif Receptor 4) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCR4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 55173751277

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4.5 ★★★★★
Based on 233 reviews
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Randy C.
Los Angeles, US
★★★★★ 5
My dog LOVES this, but...
Style: Squeaky Duck
My dog loved this right out of the box, she didn't learn to squeak it for a couple of hours, but she just stopped playing with her other toys, ragged as they may be... My dog is a Manchester/hound dog mix, of some sort, I'm only sure about the Manchester, she resembles them in look, size, weight, and all else. She weighs approximately 20-ish pounds, or so. With that said, like I said, she loves it, so much so that within 16 waking hours after its arrival she brought me the inner squeaker, the body was in the kitchen. :) She tore off the top part of the head and gained access to the squeaker, and EXACTLY as advertised and other reviews, it is a really good squeaky toy, she really doesn't seem to mind that the outer portion is gone, the (seemingly) durable squeaker might actually last. She can still play with the 'body', the toy is made that when (not if) they rip off an appendage, the nentire innards WILL NOT come out. It's a well designed toy in that respect. So, at times she will play with that too, she has brought me the BODY several times too, My dog is a really CRAZY chewer, she is somewhere under a year old and she will chew on anything that she is in front of her, including rocks, a blade of grass as she runs by, furniture legs, window sills, you name it, she has eaten it. She is a problem dog, but we picked her, and we'd do the same if it was our kid, we wouldn't throw them out. :) She has to be watched constantly, that is not the most fun, but, like they say, 'It is what it is'. I mention all of that to show that even though she is a crazy chewer, the rubber portion of the toy is still 'with us', for now, I will update this if anything else develops, and I am sure it will. Oh yeah, I really need to add, I don't think that the makers can make a chew toy 'outer' from anything that will last that long, not unless you go to a rope material, and I did not want that, it can be swallowed and cause more trouble. (Fabric can be swallowed too, but it's at least softer...) So, really, more than likely you can't get any better than this if your dog is a crazy chewer too.
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Reviewed in the United States on July 1, 2023
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Verified Purchase
Amanda Pike
Boise, US
★★★★★ 1
Awful Durability
Style: Squeaky Chicken
My dog had this toy for all of 30 minutes an COMPLETELY destroyed it. He is not an aggressive chewer, and has had plenty of rip and reveal toys from Bark Box last WEEKS. I thought I'd try out another brand, but I will be swiftly placing a Bark Box order. I am very disappointed in the durability and quality of this toy thay is supposed to survive non-agressive chewing. If I could give 0 stars, I would have.
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Reviewed in the United States on May 15, 2026
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Verified Purchase
Mrs. M
Draper, US
★★★★★ 4
Feet
Style: Squeaky Duck
Dogs love it, but tore off the feet within minutes.
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Reviewed in the United States on January 26, 2026
M
Verified Purchase
MWigles
Cuba, US
★★★★★ 5
Invincible dog toy
Style: Squeaky Duck
This dog toy is superior to all other dog toys. I got this for my 98 lb German shepherd over a year ago. It is very durable and has a LOUD squeaker. Most toys (even Kong usually) he will chew on for a few weeks but the squeaker doesn't last very long. This duck has a secondary layer that my dog finally broke into 14 months after buying and it still squeaks!!! 10/10 would recommend
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Reviewed in the United States on January 31, 2026
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Verified Purchase
TRushing
Draper, US
★★★★★ 5
Takes a licking and keeps on…you know…
Style: Squeaky Duck
Our pit is a determined chewer, and this has lasted over a week. If he annihilates it tomorrow, it was still a great success!
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Reviewed in the United States on May 4, 2026

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