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Description
Biotinylated TIM-3/HAVCR2 His&Avi Tag Protein, MouseProduct Specification Species Mouse Synonyms TIMD3, FLJ14428, KIM3 Accession Q8VIM0 1 Amino Acid Sequence Leu22 Arg191, with C terminal His and Avitag Expression System HEK293 Molecular Weight 35 55kDa (Reducing) Purity 95% by SDS PAGE Endotoxin <1EU g Physical Appearance Lyophilized Powder Storage Buffer PBS, pH7. 4. Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid centrifugation. Stability & Storage 12
Product Specification
| Species | Mouse |
| Synonyms | TIMD3, FLJ14428, KIM3 |
| Accession | Q8VIM0-1 |
| Amino Acid Sequence | Leu22-Arg191, with C-terminal His and Avitag |
| Expression System | HEK293 |
| Molecular Weight | 35-55kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345. 2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213. |
Background
TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region. Mature mouse TIM-3 consists of a 174 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic tail. Within the ECD, mouse TIM-3 shares 58% and 75% aa sequence identity with human and rat TIM-3, respectively. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. The TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor.
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