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Description
Human ATGL ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes and remove the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the reagent kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 20ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with adipose triglyceride lipase (ATGL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of adipose triglyceride lipase (ATGL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human adipose triglyceride lipase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Adipose triglyceride lipase, also known as protease domain-containing protein 2 and ATGL, is an enzyme encoded by the PNPLA2 gene. ATGL catalyzes the first reaction in lipolysis, in which triacylglycerols are hydrolyzed to diacylglycerols. ATGL has a very high substrate specificity for triacylglycerols and contains a catalytic dyad that utilizes a serine-aspartate nucleotide. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenate, cell culture supernatant |
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★★★★★ 5
Love it
Size: Queen, Color: White
This is such a nice pillow protector. It is super soft, the zipper works great, it has a decent weight to it, and definitely keeps the pillow cooler. I’m very impressed.
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Reviewed in the United States on April 14, 2026
★★★★★ 5
Great product! I love the feel of these.
Size: Queen, Color: White
“I recently purchased the Serta Power Chill Cooling Pillow Protectors, and they have been a game-changer for my sleep comfort. These pillow protectors have exceeded my expectations in several ways.
First and foremost, the cooling technology is fantastic. I tend to sleep hot, especially during the summer months, and these pillow protectors have made a noticeable difference. They keep my pillows cool and comfortable throughout the night, which has led to more restful and uninterrupted sleep.
The fit is also excellent. They easily slip over my standard-sized pillows and stay securely in place without any annoying bunching, wrinkles or slipping during the night. And the zipper is amazing. Having no wrinkles in my cover is easier on my hair.
What’s great about these protectors is that they not only keep my pillows cool but also protect them from spills, stains, and allergens. It gives me peace of mind knowing that my pillows are safeguarded while I enjoy a cool and refreshing sleep environment. There is no noise when sleeping on this cover, it’s soft.
Cleaning is a breeze. They are machine-washable and maintain their cooling properties even after multiple washes.
The quality of the materials used is evident. These protectors feel durable and well-made, promising long-lasting use.
While they are slightly more expensive than basic pillow protectors, the added cooling benefits and protection they offer are well worth the investment. They’ve made a significant difference in my sleep quality, and I highly recommend the Serta Power Chill Cooling Pillow Protectors to anyone seeking a cooler and more comfortable night’s sleep.”
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Reviewed in the United States on October 6, 2023
★★★★★ 4
Loose on standard pillow.
Size: Queen, Color: White
Too large for standard pillow. It would fit a queen size pillow much better. It does stay cool.
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Reviewed in the United States on November 20, 2025
★★★★★ 5
Soft and cool. Very cool. 🧊
Size: Queen, Color: White
I bought these for my memory foam pillow and they’re excellent for cooling it. I use 2 of these on the pillow for maximum chill factor and it works.
They’re very soft, the zippers are high quality. Easy to put on with a bit of stretch- generously sized. They’re stain resistant. I’m glad they’re NOT waterproof because they’d be too hot then. They’re very durable. Wash on delicate setting in a mesh bag and air dry and they last long. They’re very quiet- smooth and silky. Definitely recommend!
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Reviewed in the United States on January 15, 2026
★★★★★ 3
Cool enough
Size: Queen, Color: White, Size: Queen, Color: White
I thought I had purchased pillows so when I received these I was a little disappointed. However that was 100% on me for not reading the listing and just trusting my search. I decided to give them a try and so far they seem ok. They don’t stay cool even though I have them on cooling pillows. They are super thin but very soft. They fit on my pillows perfectly. I don’t think it has improved my sleep quality but it hasn’t made it any worse either. I don’t think they are waterproof but I don’t really want to test that. I like that the zipper is small and seems to be decent quality. Smelled heavily of chemicals when I first took it out of the package but that disappeared after a few minutes.
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Reviewed in the United States on March 21, 2026