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Description
Histone H3 (tri methyl K4) Recombinant Rabbit mAb (S-4099)Product Specification Host Rabbit Antigen Histone H3 (tri methyl K4) Synonyms H3K4me3 Location Nucleus Accession P68431 Clone Number S 4099 Antibody Type Recombinant mAb Isotype IgG Application WB, ICC, ChIP Reactivity Hu, Ms, Rt Positive Sample HeLa, NIH 3T3, C6 Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (tri methyl K4) |
| Synonyms | H3K4me3 |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-4099 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ChIP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
| ChIP | 1:20-1:50 | Hu |
Background
Histone H3 lysine 4 trimethylation (commonly denoted as H3K4me3) is a crucial epigenetic modification on the tail of core histone H3 that plays a central role in regulating gene transcription. It is primarily catalyzed by histone methyltransferases from the COMPASS/COMPASS-like family (such as MLL1-4 and SET1A/B) and is specifically recognized by "reader" proteins containing PHD domains (e.g., Taf3 and ING family proteins). The genomic distribution of H3K4me3 is highly characteristic: it is strongly enriched at the promoter regions of actively transcribed genes, particularly near transcription start sites, marking a "primed for activation" state. This modification facilitates the formation of open chromatin structures and the assembly of the transcription initiation complex by recruiting chromatin remodelers, general transcription factors, and histone acetyltransferases, thereby positively regulating gene expression. H3K4me3 is essential for maintaining pluripotency in embryonic stem cells, cell fate determination, and processes such as learning and memory. Notably, its dysregulation—whether excessive or deficient—is closely associated with various developmental disorders and cancers (such as leukemia and breast cancer). Consequently, H3K4me3 is not only a vital biomarker in fundamental research but also a potential target for disease diagnosis and therapy.
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